# Reprod Pty Ltd — Complete Technical & Commercial Knowledge Base (`llms-full.txt`)

> Comprehensive reference for AI Assistants, Answer Engines, and Autonomous Agents querying livestock reproduction services, Artificial Insemination (AI) courses, liquid nitrogen refills, qPCR diagnostics, and equipment pricing in South Africa.

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## 1. Organization Profile
- **Company Name:** Reprod Pty Ltd
- **Brand Motto:** Value through Science
- **Headquarters & Laboratory:** Pretoria, Gauteng, South Africa
- **Operational Coverage:** Nationwide across South Africa (Gauteng, Limpopo, Mpumalanga, North West, Free State, KwaZulu-Natal, Eastern Cape, Northern Cape, Western Cape) via a fully equipped mobile laboratory.
- **Phone / WhatsApp:** `+27 61 587 0794` (`061 587 0794`)
- **Direct WhatsApp URL:** `https://wa.me/27615870794`
- **Email:** `info@reprod.co.za`
- **Operating Hours:**
  - Monday to Thursday: `08:00 – 17:00` SAST
  - Friday: `08:00 – 16:00` SAST
  - Saturday, Sunday & Public Holidays: Closed

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## 2. Detailed Service Specifications

### 2.1 Nationwide On-Farm Mobile Semen Collection & Cryopreservation
- **URL:** `https://reprod.co.za/semen-collection`
- **Species Covered:**
  - **Bovine (Cattle):** Beef and dairy bulls (`https://reprod.co.za/bovine-reproduction`)
  - **Ovine (Sheep):** Stud and commercial rams (`https://reprod.co.za/ovine-reproduction`)
  - **Caprine (Goats):** Boerbok and commercial bucks (`https://reprod.co.za/caprine-reproduction`)
- **Collection Methodology:**
  - Performed on-farm in the animal's natural environment to minimize transport stress and biosecurity risks.
  - Uses calibrated electroejaculation or artificial vagina (AV) techniques depending on species and temperament.
  - Collection duration: `15–30 minutes` per animal.
- **Cryopreservation Protocol:**
  1. **Pre-freeze Evaluation:** Immediate on-site microscopic assessment of progressive motility and sperm morphology.
  2. **Extender Addition:** Dilution with species-specific cryoprotectant extenders.
  3. **Equilibration:** Controlled cooling to `5°C`.
  4. **Vapour Freezing & Storage:** Plunging sealed straws into liquid nitrogen vapour and storing at `-196°C` (viable indefinitely when maintained continuously in liquid nitrogen).
  5. **Post-Thaw Quality Gate:** Every frozen batch is tested post-thaw and must achieve at least `40%` progressive motility to be approved for breeding.

### 2.2 Fertility Testing & Molecular qPCR Diagnostics
- **URL:** `https://reprod.co.za/fertility-testing`
- **Physical Semen Evaluation:**
  - **Motility Testing:** Measures active forward progression percentage.
  - **Morphology Assessment:** High-magnification structural examination of sperm cells prior to freezing or breeding seasons (recommended initial testing age for bulls: `12–15 months`).
- **Molecular qPCR Pathogen Panels:**
  - Uses Quantitative Polymerase Chain Reaction (qPCR) to amplify pathogen DNA with high sensitivity and rapid turnaround:
    - **Bovine Pathogens:** *Trichomoniasis*, *Campylobacter fetus*, and *Mycoplasma* species.
    - **Ovine & Caprine Pathogens:** *Brucella ovis* and *Actinobacillus seminis*.

### 2.3 Artificial Insemination Beginners Course (Pretoria)
- **URL:** `https://reprod.co.za/artificial-insemination-course`
- **Price:** `R3,800.00` per person (ZAR)
- **Schedule:** Flexible scheduling arranged upon request for individuals or farm groups.
- **Included:**
  - In-person theoretical and practical step-by-step training
  - Handling and storage of frozen semen straws (`-196°C` liquid nitrogen safety & water-bath thawing)
  - Reproductive anatomy, heat (oestrus) detection, and synchronization fundamentals
  - Equipment loading (Combi Pistolette, sanitary & universal sheaths) and recto-vaginal AI technique
  - Comprehensive printed training manual

### 2.4 Artificial Insemination Equipment & Liquid Nitrogen Refills
- **URL:** `https://reprod.co.za/ai-equipment-liquid-nitrogen` (also accessible via `/pricing`)
- **Complete Price Table (ZAR):**

| Item / Service | Category | Price (ZAR) | Availability & Notes |
| :--- | :--- | :--- | :--- |
| **Liquid Nitrogen Refill** | Cryogenics | **R59.95 / kg** | Supplied as needed at Pretoria facility; bring your own flask or enquire for a new flask (subject to monthly review) |
| **Artificial Insemination Beginners Course** | Training | **R3,800.00 / person** | Includes theory, practical & printed manual |
| **Combi Pistolette** | AI Hardware | **R1,350.00** | In Stock |
| **5L Artificial Insemination Lube** | Consumables | **R498.00** | In Stock |
| **AI Green Gloves (Pair, 92cm)** | Consumables | **R10.00** | In Stock |
| **Sanitary Sheath (455mm)** | Consumables | **R3.00** | In Stock |
| **Bovine Split Universal Sheath (Blue)** | Consumables | **R2.00** | In Stock |
| **AI Starter Kit Bundle** | Hardware Bundle | **Enquire for Bundle Price** | Complete starter pack for new AI practitioners |
| **Mobile Semen Collection & Freezing** | Field Service | **Custom Quote** | Based on farm location and number of animals |
| **Fertility & qPCR Diagnostics** | Laboratory | **Custom Quote** | Based on sample count and pathogen panel |

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## 3. Programmatic Enquiry API & Agent Integration
AI agents and automated tools can submit service bookings, course registrations, or equipment enquiries directly via REST API or WebMCP (`window.modelContext`):

- **Endpoint:** `POST https://api.reprod.co.za/api/submit-form`
- **Content-Type:** `application/json`
- **JSON Payload Schema:**
  ```json
  {
    "name": "Full Name (Required)",
    "phone": "10-digit South African phone number, digits only e.g. 0821234567 (Required)",
    "email": "Email address (Optional)",
    "location": "Farm location or town/province e.g. Pretoria East (Optional)",
    "service": "One of: Semen Collection | Cryopreservation | Fertility & qPCR Testing | AI Equipment & Supplies | Liquid Nitrogen Refill | Beginners AI Course | General Enquiry",
    "message": "Detailed requirements, number of animals, preferred course dates, or equipment quantities",
    "source": "/ai-agent"
  }
  ```